SAGITTA: the advantages of an innovative approach to a consolidated technology
SAGITTA represents a sophisticated and innovative approach to PCR that offers a high degree of multiplexing that can also be performed starting PCR amplification directly from briefly pre-treated raw samples, allowing up to 20 different targets to be detected and differentiated in a single well.
SAGITTA overcomes the current limitations of the most common tests on the market, such as the high cost and limited multiplex capability (for PCR tests based on the use of probes) and low specificity (for PCR tests based on DNA intercalators), offering highly multiplexed analysis while delivering unbeatable accuracy.
The melting curve analysis, readable through a single fluorescence channel by the most common PCR instruments, generates distinctive melting temperatures for each single target present in the sample. This allows SAGITTA to detect up to 20 target analyzes, without losing specificity precisely because each fingerprint is unique and distinguishable.
Not only SAGITTA is highly informative, but also the analysis workflow is faster: the diagnostic analysis can be carried out directly without any pre-analytic steps aimed to perform DNA/RNA extraction and purification, thus saving time and money . This occurs through the use of a specific reagent developed by Ulisse BioMed called Ulisse Faster.